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1.
Rev. Ateneo Argent. Odontol ; 66(1): 48-51, 2022.
Article in Spanish | LILACS | ID: biblio-1380370

ABSTRACT

Los dientes animales de las diferentes especies (hu- mano, equino, cerdo, etc.) están constituidos histológi- camente por cuatro tejidos fundamentales. Ellos son: esmalte, dentina, cemento y pulpa dental. Su compo- sición, estructura, morfología y tamaño son disímiles para cada género. Según numerosas investigaciones, los dientes de bovino serían los de elección por ser de fácil obtención y por tener muy pocas, o ninguna, diferencias tanto a nivel macro como microscópico con respecto a los dientes humanos. El objetivo de la presente revisión es aportar información actualizada acerca de las características histológicas de los tejidos dentarios bovinos y profundizar el conocimiento de las similitudes y diferencias de los dientes bovinos y humanos dando soporte a otros estudios compa- rativos y promoviendo la utilización de las piezas dentarias bovinas en trabajos de investigación en odontología (AU)


The animal teeth of the different species (human, equine, pig, etc.) are histologically constituted by four fundamental tissues: enamel, dentin, cement and dental pulp. Their composition, structure, morphology and size are dissimilar for each gender. According to numerous investigations, bovine teeth would be the ones of choice because they are easy to obtain and have very few or no differences, both at the macro and microscopic levels, with respect to human teeth. The objective of this review is to provide updated information about the histological characteristics of bovine dental tissues and deepen the knowledge of the similarities and differences between bovine and human teeth, supporting other comparative studies and promoting the use of bovine dental pieces in research work in dentistry (AU)


Subject(s)
Humans , Animals , Cattle , Tooth/anatomy & histology , Cattle , Dental Research , Dental Cementum/anatomy & histology , Dental Enamel/anatomy & histology , Dental Pulp/anatomy & histology , Dentin/anatomy & histology
2.
Belo Horizonte; s.n; 2019. 138 p. ilus, tab.
Thesis in English, Portuguese | LILACS, BBO | ID: biblio-1016737

ABSTRACT

Dentes bovinos têm sido utilizados como substitutos aos humanos em pesquisas odontológicas. Entretanto, faltam dados que suportem o uso da dentina radicular bovina, sobretudo, que analisem o efeito da idade nas propriedades do substrato. Assim, analisou-se o efeito da idade nos aspectos morfológicos, químicos e físicos das dentinas radiculares humana e bovina, em função dos terços da raiz. Trata-se de um estudo experimental, in vitro, aprovado pelo Comitê de Ética em Pesquisa (COEP-UFMG 1.803.933) e pela Comissão de Ética no Uso de Animais (CEUAUFMG 372/2016). A amostra foi composta por dentes unirradiculares, cujas dentinas radiculares foram categorizadas em: humana jovem (HJ, 20-30 anos); humana madura (HM, acima de 60 anos); bovina jovem (BJ, 24-36 meses); e bovina adulta (BA, acima de 48 meses). Os dentes foram seccionados abaixo da junção amelocementária e ao longo eixo da raiz, para a obtenção de hemisecções. Uma hemisecção foi escolhida e cortes foram feitos para a obtenção dos espécimes, conforme cada método de análise. Para as análises dos aspectos morfológicos ­ número, diâmetro e área dos túbulos dentinários ­ os espécimes foram analisados em Microscópio Eletrônico de Varredura. A composição química foi analisada por Espectroscopia de Raios X por Dispersão em Energia, Espectroscopia por Comprimento de Onda Dispersivo e Espectroscopia Raman por Transformada de Fourier. Na análise das propriedades mecânicas, os espécimes foram submetidos aos testes de nanoindentação, resistência à flexão em três pontos e compressão. Para a análise da resistência ao cisalhamento por extrusão, as raízes receberam tratamento endodôntico e foram fixados pinos de fibra de vidro com cimentos resinosos (dual convencional e autoadesivo). As raízes foram seccionadas nos terços radiculares e os espécimes testados em máquina universal de ensaios. Adicionalmente, avaliou-se o grau de conversão dos cimentos resinosos. Os dados foram analisados em software estatístico, nível de significância de 5%. A HM apresentou os menores valores para número, diâmetro e área de túbulos dentinários (p<0,05). A HM apresentou maiores valores de cálcio, razão Ca/P e concentração mineral relativa, com diminuição na organização e qualidade do colágeno (p<0,05). A HM apresentou os maiores valores de nanodureza, módulo de elasticidade e resistência à compressão, mas os menores valores de resistência à flexão e módulo de elasticidade à flexão (p<0,05). A HM apresentou os menores valores de resistência de união, com diferenças entre os terços radiculares (p<0,05). O grau de conversão dos cimentos apresentou uma diminuição ao longo dos terços radiculares (p<0,05). Concluiu-se que a idade possui um efeito nos aspectos morfológicos, químicos e físicos da dentina radicular humana, sem efeito na dentina bovina. Assim, quando da necessidade de substituição da dentina radicular humana pela bovina em pesquisas, é necessário considerar o efeito da idade nos desfechos analisados.


Bovine teeth have been used as a substitute for human teeth in dental research. However, there is a lack of data that support the use of bovine root dentin, mainly, that analyze the effect of age on the properties of the substrate. Thus, the effect of age on the morphological, chemical and physical aspects of human and bovine root dentin was analyzed, in function of the root thirds. This is an in vitro experimental study, approved by the Research Ethics Committee (protocol number: 1.803.933) and the Ethics Committee on the Use of Animals (protocol number: 372/2016). The sample was composed of single-rooted teeth and root dentin was categorized into: young human (YH, 20-30 years); old human (OH, above 60 years); young bovine (YB, 24-36 months); and adult bovine (AB, over 48 months). The roots were sectioned below the cement-enamel and longitudinally to produce two hemi-sections. One hemi-section was chosen and sections were made to obtain the specimens, according to each method of analysis. For the analyzes of the morphological aspects - number, diameter and area of the dentinal tubules - the specimens were analyzed in Scanning Electron Microscope. The chemical composition was analysed by Energy Dispersive Spectroscopy, Wavelength Dispersive Spectroscopy and Fourier Transformed Raman Spectroscopy. In the analysis of the mechanical properties, the specimens were submitted to nano-indentation, three-point flexural and compression tests. For the analysis of the push-out bond strength test, the roots received endodontic treatment and fiber post with resin cements (conventional and selfadhesive dual) were fixed. The roots were sectioned in the root thirds and the specimens tested in a universal testing machine. Additionally, the degree of conversion of the resin cements was evaluated. The data were analyzed in statistical software, level of significance of 5%. The OH presented the lowest values for number, diameter and area of dentinal tubules (p<0.05). OH showed higher values of calcium, Ca/P ratio and relative mineral concentration, with decrease in the organization and quality of the collagen (p<0.05). The OH showed the highest values of nano-hardness, elastic modulus and compressive strength, but the lowest values of flexural strength and flexural modulus (p<0.05). The OH presented the lowest values of bond strength, with differences among the root thirds (p<0.05). The degree of conversion of the cements showed a decrease along the root thirds (p<0.05). It was concluded that age has an effect on the morphological, chemical and physical aspects of human root dentin, with no effect on bovine dentin. Thus, when it is necessary to replace the human root dentin by the bovine in research, it is necessary to consider the effect of age on the analysed outcomes.


Subject(s)
Tooth Root , Resin Cements , Dental Pins , Dentin/anatomy & histology , Fractures, Compression , Flexural Strength , Hardness Tests , In Vitro Techniques , Microscopy, Electrochemical, Scanning , Microscopy
3.
RFO UPF ; 23(1): 12-16, 15/08/2018. tab, ilus
Article in English | LILACS, BBO | ID: biblio-910057

ABSTRACT

Objective: to verify the accuracy of cone beam computedtomography (CBCT) for measuring dentin thicknessin the danger zone of mandibular molars in order to validatethis method to be used as a clinical auxiliary. Materialsand method: dentin thickness of the distal wall ofmesial canals was measured at 2 mm of furcation areain ten mandibular molars before and after preparation ofcervical and middle thirds. CBCT (0.25 mm voxel size)and stereomicroscopy images were acquired twice foreach sample. CBCT axial images and stereomicroscopeimages were evaluated by a calibrated examiner. Statisticalanalysis was performed using Mann-Whitney testin order to investigate whether CBCT images providedtrusted values. Results: mean values of initial (1.23 versus1.24 mm) and final measurements (0.88 versus 0.87mm) obtained with, respectively, CBCT and stereomicroscopemethods did not show statistical differences(P > 0.5). Conclusion: CBCT images provide high accuracyfor measuring dentin thickness in the danger zoneof mandibular molars. (AU)


Objetivo: avaliar a acurácia da tomografia computadorizada de feixe cônico (TCFC) para mensurar a espessura dentinária na zona de risco de molares inferiores e validar este método, a fim de usá-lo como um auxiliar clínico. Materiais e método: a espessura dentinária da parede distal dos canais mesiais foi medida a 2 mm da área de furca em dez molares inferiores, antes e após a preparação dos terços médio e cervical. Foram adquiridas imagens por TCFC (tamanho do voxel de 0,25 mm) e estereomicroscopia. Um examinador treinado e calibrado avaliou as tomografias no plano axial e as imagens de estereomicroscopia. A análise estatística foi realizada com o teste Mann-Whitney, para verificar se as imagens tomográficas forneceram valores comparáveis à estereomicroscopia. Resultados: a média dos valores obtidos, respectivamente, após as mensurações iniciais (1,23 versus 1,24 mm) e finais (0,88 versus 0,87 mm) das imagens de TCFC e estereomicroscopia não mostraram diferença estatisticamente significativa entre os métodos (P > 0,5). Conclusão: a TCFC produz imagens acuradas para a mensuração da espessura dentinária na zona de risco de molares inferiores. (AU)


Subject(s)
Humans , Dentin/diagnostic imaging , Cone-Beam Computed Tomography/methods , Molar/diagnostic imaging , Reference Values , Image Processing, Computer-Assisted/methods , Statistics, Nonparametric , Dentin/anatomy & histology , Dimensional Measurement Accuracy
4.
Rev. ADM ; 74(3): 127-132, mayo-jun. 2017. tab, graf
Article in Spanish | LILACS | ID: biblio-908009

ABSTRACT

La estimación de la edad cronológica en el estudio de restos humanos esuna tarea esencial para llegar a conclusiones más objetivas respecto a la persona estudiada, la precisión en el cálculo de este dato es un componentefundamental en estudios en contextos antropológicos y forenses. Una alternativa es la que se basa en la medición de la transparencia dela dentina radicular de los dientes permanentes. En el presente estudiose pone a prueba una técnica de estimación de la edad basada en lamedición de la transparencia radicular de dientes procedentes de unacolección esquelética de edad conocida. Los dientes incluidos en este estudiofueron unirradiculares, de la segunda dentición y completos en su estructura. Fueron recolectados de restos esqueletizados desenterrados de un cementerio contemporáneo del poblado de Caltimacán, Hidalgo. Los dientes fueron preparados para favorecer el paso de un haz de luz incandescente a través de su raíz. La medición de la transparencia se llevó a cabo con un calibrador digital con precisión de lectura de centésimas de milímetro. Resultados: El coeficiente de correlación entre la edad conocida y la edad estimada con base en la tabla de predicción de Bang y Ramm (1970) es alto, mostrando un índice de 0.75. En este estudio la diferencia media en años de la estimación que representaun parámetro importante para establecer la efectividad de una técnicafue de −3.06, lo que significa que las predicciones subestiman la edadreal. Conclusiones: El empleo de la transparencia radicular muestrabondad en la estimación, aunada a la sencillez en la observación, aunqueimplica necesariamente la disposición de dientes fuera de su alveolo


Subject(s)
Male , Female , Humans , Young Adult , Adult , Middle Aged , Aged , Age Determination by Teeth/methods , Dentin/anatomy & histology , Forensic Anthropology/methods , Tooth Root/anatomy & histology , Age and Sex Distribution , Body Remains/anatomy & histology , Forensic Dentistry/methods , Mexico , Data Interpretation, Statistical
5.
J. appl. oral sci ; 25(1): 27-33, Jan.-Feb. 2017. tab, graf
Article in English | LILACS, BBO | ID: biblio-841159

ABSTRACT

Abstract Objective This study aimed to evaluate the influence of the NiTi wire in Conventional NiTi (ProTaper Universal PTU) and Controlled Memory NiTi (ProTaper Gold PTG) instrument systems on the quality of root canal preparation. Material and Methods Twelve mandibular molars with separate mesial canals were scanned using a high-definition microcomputed tomography system. The PTU and PTG instruments were used to shape twelve mesial canals each. The canals were scanned after preparation with F2 and F3 instruments of the PTU and PTG systems. The analyzed parameters included the remaining dentin thickness at the apical and cervical levels, root canal volume and untouched canal walls. Data was analyzed for statistical significance by the Friedman and Dunn’s tests. For the comparison of data between groups, the Mann-Whitney test was used. Results In the pre-operative analysis, there were no statistically significant differences between the groups in terms of the area and volume of root canals (P>.05). There was also no statistically significant difference between the systems with respect to root canal volume after use of the F2 and F3 instruments. There was no statistical difference in the dentin thickness at the first apical level between, before and after instrumentation for both systems. At the 3 cervical levels, the PTG maintained centralization of the preparation on the transition between the F2 and F3 instruments, which did not occur with the PTU. Conclusion The Conventional NiTi (PTU) and Controlled Memory NiTi (PTG) instruments displayed comparable capabilities for shaping the straight mesial root canals of mandibular molars, although the PTG was better than the PTU at maintaining the centralization of the shape in the cervical portion.


Subject(s)
Humans , Titanium/chemistry , Root Canal Preparation/instrumentation , Dental Alloys/chemistry , Dental Instruments , Nickel/chemistry , Reference Values , Surface Properties , Materials Testing , Reproducibility of Results , Statistics, Nonparametric , Root Canal Preparation/methods , Dental Pulp Cavity/anatomy & histology , Dentin/anatomy & histology , Equipment Design , X-Ray Microtomography , Molar
6.
Int. j. morphol ; 34(2): 597-603, June 2016. ilus
Article in English | LILACS | ID: lil-787042

ABSTRACT

The structural changes and morphological derivatives of progressive dentin hypermineralization determine the bonding to its surface. A planimetric study and fracture volume estimate were conducted to assess the efficacy of resin composite bonded to hypermineralized cervical dentin with and without retentions by applying a shear test. Thirty-four premolars from adult patients were used to obtain a flat cervical dentin surface. The specimens were distributed randomly into two groups: 1) experimental, where hemispherical retentions were carved into the cervical dentin, and 2) control, where the flat dentin was maintained. Using a jig, a resin column was built into the dentin surface fitted with bonding in specimens from both groups, which were then subjected to the shear test. The morphological analysis was performed with a microscope while the percentage of remnant surface from each component of the adhesive bond was calculated planimetrically. The volume of lost tissue was determined using Scherle's method. In the planimetric study, the average remnant resin on the surface was significantly higher in the experimental group and there was less intact dentin in the control group. The volumetric assessment also showed a much higher loss of dentin substrate than the experimental group. Consequently, the complementary use of morphometric and volumetric techniques enables the evaluation of the efficacy of a technique, demonstrating that the inclusion of mechanical retentions in hypermineralized cervical dentin improves the bonding properties.


Los cambios estructurales y morfológicos derivados de la hipermineralización dentinaria progresiva determina la adhesión a su superficie. Fue realizado un estudio planimétrico y el volumen de fractura estimado para evaluar la eficacia de resina compuesta adherida a dentina cervical hipermineralizada con y sin retenciones a través de la prueba de cizalla. Treinta y cuatro premolares obtenidos de pacientes adultos fueron utilizados para obtener una superficie dentinaria cervical plana. Las muestras fueron distribuidas aleatoriamente en dos grupos: 1) experimental, donde se realizaron retenciones hemiesféricas a la dentina cervical, y 2) control, donde la dentina plana fue mantenida. Usando un dispositivo, una columna de resina fue construida en la superficie dentinaria y adaptada con adhesivos en las muestras de ambos grupos, las cuales fueron sometidas al test de cizalla. El análisis morfológico fue realizado con un microscopio mientras que el porcentaje de superficie remanente para cada componente del adhesivo fue calculado planimetricamente. El volumen del tejido perdido fue determinado utilizado el método de Scherle. En el estudio planimétrico, el promedio de resina remanente en la superficie fue significativamente mayor en el grupo experimental y con menos dentina intacta en el grupo control. El análisis volumétrico mostró una mayor perdida de sustrato dentinario en el grupo control. De esta manera, el uso de técnicas morfométricas y volumétricas permite la evaluación de la eficacia de una técnica, demostrando que la inclusión de retenciones mecánicas en la dentina hipermineralizada cervical mejora las propiedades de adhesión.


Subject(s)
Humans , Composite Resins , Dentin-Bonding Agents , Dentin/anatomy & histology , Odontometry/methods
7.
São Paulo; s.n; 2016. 103 p. ilus, tab. (BR).
Thesis in Portuguese | LILACS, BBO | ID: biblio-867993

ABSTRACT

A presente pesquisa se propôs a avaliar a influência de cinco tratamentos dentinários na resistência de união à dentina de quatro cimentos de ionômero de vidro modificados por resina (CIVRMs). O ensaio experimental foi realizado in vitro, fazendo uso de 200 incisivos bovinos. Destes, foram obtidos 200 fragmentos de dentina, que foram distribuídos aleatoriamente em 20 grupos (n=10), segundo os fatores de variação que compreendem cinco tratamentos dentinários (Grupo controle, ou seja, sem tratamento; Ácido Poliacrílico a 20%, Clearfil SE Bond; Single Bond Universal e Primer do Vitremer) e quatro cimentos ionoméricos (GC Fuji II LC® CAPSULE; GC Fuji LINING® LC (Paste Pak); Vitrebond® e Vitremer). Após a confecção dos corpos de prova e 24h de armazenamento em estufa à 37oC, foi realizado o teste de microcisalhamento na máquina de ensaios universal (Instron 5942 - Canton, MA, EUA) com velocidade de 1mm/min, seguido da análise do padrão de fratura em microscópio digital. A análise estatística dos 400 resultados obtidos foi feita por meio do teste paramétrico ANOVA para dois fatores de variação (cimento e tratamento) (p<0,05%) e pelo teste auxiliar de Tukey (p<0,05%). Os testes estatísticos demonstraram haver diferença estatística tanto entre os tratamentos, como entre os cimentos e na interação de ambos (p<0,0001). Os sistemas adesivos Clearfil SE (X?=18,26 ± 2,78) e SingleBond (X?=17,28 ± 2,49) promoveram os valores de resistência adesiva mais elevados em comparação aos demais tratamentos, mas sem apresentarem


diferença estatística entre si, o que representou um aumento na resistência de união de 121% para o Clearfil SE e 109,2% para o Single Bond Universal em relação ao Grupo Controle. O uso do Ácido Poliacrílico (X?=9,94 ± 3,55) alcançou valores intermediários, correspondendo a um aumento de 20% na resistência adesiva em relação ao Grupo Controle. Esse tratamento se diferenciou estatisticamente dos maiores valores, porém não apresentou diferença frente ao Primer do Vitremer (X?=8,91 ± 2,97). O grupo controle (X?=8,26 ± 4,17) evidenciou os menores valores, se diferenciando estatisticamente dos sistemas adesivos e do Ácido Poliacrílico, mas com resultados que o equiparou ao tratamento com o Primer do Vitremer. Ao se analisar a interação entre cimento e tratamento, todos os cimentos de ionômero de vidro resinomodificados (CIVRMs) testados neste estudo apresentaram seus maiores resultados quando um dos sistemas adesivos foi utilizado como tratamento dentinário, independente de qual sistema adesivo. Com base nesses resultados, pôde-se concluir que os dois sistemas adesivos autocondicionantes foram capazes de melhorar significativamente a resistência adesiva dos CIVRMs.


This research aims to evaluate the influence of five dentin pretreatments on microshear bond strength of four resin-modified glass ionomer (RMGI). The experiment was performed in vitro, using 200 bovine incisors. From these, there were obtained 200 dentine fragments that were randomly distributed into 20 groups (n = 10) according to the variation factors comprising five dentin treatment (control group, i.e., no treatment; Polyacrylic Acid 20%; Clearfil SE Bond; Single Bond Universal and Vitremer Primer) and four glass ionomer cements (GC Fuji II LC® CAPSULE; GC Fuji LC LINING® (Paste Pak); Vitrebond® and Vitremer). After preparation of the samples and 24h storage at 37 °C, was performed microshear test in a universal testing machine (Instron 5942 - Canton, MA, USA) with speed of 1mm/min, followed by analysis of the pattern fractures in digital microscope. Statistical analysis of the 400 results was performed by ANOVA nonparametric test for two variation factors (cement and treatment) (p <0.05%) and the post-hoc Tukey (p <0.05%). The tests demonstrated there are statistical difference between treatments, cements and between the interaction of both (p <0.0001). The Clearfil SE (X?=18,26 ± 2,78) and SingleBond (X?=17,28 ± 2,49) promoted the highest bond strength values compared to the other treatments, but showing no statistical difference between them, which represented an increase in bond strength of 121% for


Clearfil SE and 109.2% for Single Bond Universal compared to control group. The Polyacrylic Acid (X?=9,94 ± 3,55) has reached intermediate values, corresponding to a 20% increase in adhesive strength compared to control group. This treatment differed significantly from the higher values, but showed no difference compared to Primer Vitremer (X?=8,91 ± 2,97). The control group (X?=8,26 ± 4,17) showed the lowest values, statistically differing from the adhesives and polyacrylic acid, but with results that matched to the treatment with Vitremer Primer. When analyzing the interaction between cement and treatment, all cements tested in this study had higher results when one of the adhesive systems was used as dentin treatment, regardless of which adhesive system. Based on these results, it could be concluded that the two adhesive systems were able to significantly improve the adhesive strength of RMGIs.


Subject(s)
Adhesives , Dentin/anatomy & histology , Materials Science , Glass Ionomer Cements
8.
Braz. oral res. (Online) ; 29(1): 1-9, 2015. tab, ilus
Article in English | LILACS | ID: lil-777169

ABSTRACT

This study aimed to guide the planning of anticurvature filing using pre-determined anatomical points on teeth to establish directions for proper implementation of the technique. Two hundred digital periapical radiographs of human molar teeth were selected and divided into two groups (n = 100): MX (maxillary) and MD (mandibular) molars. Mesiobuccal roots were considered for the MX group and mesial roots for the MD group. Pre-determined anatomical points required for planning the anticurvature filing on the root canal path were located, and the distances between these points obtained. The anticurvature filing was simulated in two different protocols for each group, and the region of dentin removal and the remaining dentin thickness were measured in the safety and danger zones of the root canals. Statistical analysis was carried out at a significance level of 5%. The distances between the anatomical points and the thickness of remaining dentin showed significant differences when the two groups were compared (p < 0.001). No significant differences were found between the two experimental groups regarding the area of dentin removal at the root region, but differences were detected in comparison with dentin removal at the crown (p < 0.001). In terms of wear produced after simulation of both anticurvature filing protocols, significant differences were verified for all regions, except for the dentin remaining at the danger zone. The radiographic location of anatomical points allows for planning and implementation of controlled and efficient anticurvature filing and can be performed in the same manner for maxillary and mandibular molars.


Subject(s)
Humans , Filing , Molar/anatomy & histology , Molar , Root Canal Preparation/methods , Tooth Root/anatomy & histology , Tooth Root , Analysis of Variance , Anatomic Landmarks , Dentin/anatomy & histology , Dentin , Odontometry/methods , Reference Values , Reproducibility of Results , Statistics, Nonparametric
9.
Int. j. morphol ; 32(3): 956-961, Sept. 2014. ilus
Article in Spanish | LILACS | ID: lil-728294

ABSTRACT

En una investigación forense existen diversas incógnitas médico legales y criminalísticas por definir: especie, sexo, edad, identidad, causa y data de muerte de la víctima o victimario. Fenómenos biológicos han colaborado en estimar la edad médico legal de un individuo. Investigadores han propuesto diferentes métodos para esta estimación. El fenómeno de la translucidez dentinaria radicular se asocia con el aumento de la edad del individuo y ha demostrado ser de gran utilidad en esta estimación. El objetivo fue comparar el acierto en la estimación de la edad médico legal en tres rangos etarios predeterminados, mediante la medición de la translucidez dentinaria radicular, aplicando dos metodologías. Se realizaron mediciones de la longitud de la translucidez de piezas dentarias de una población chilena (n=94), mediante un método visual directo convencional y un método digital indirecto basado en una captura computarizada de la imagen. Se estimó la edad aplicando una tabla propuesta. Se preestablecieron tres rangos de edad: 1er rango (20-39 años), 2do rango (40­59 años) y 3er rango (60­79 años). El método digital muestra un acierto en la estimación de la edad en el 1er rango etario (93%), en comparación al método visual que muestra un acierto 2do y 3er rango (86% y 65%). Existen diferencias significativas p=0,001 entre ambos métodos para cada rango. La diferencia no es estadísticamente significativa en los porcentajes totales de aciertos en la estimación de la edad con ambos métodos. Enfrentados a la estimación de la edad en piezas dentarias con poca TDR se recomienda el método digital, considerando que tiende a una subestimación. En casos de mayor TDR recomendamos el método visual, que tiende a sobreestimar. Este trabajo demuestra que ambos métodos son igualmente efectivos.


In a forensic investigation there are several forensic and criminology unknowns to be elucidated, such as, species, sex, age, identity, cause and date of death of the victim or perpetrator. Some biological phenomena have been used to collaborate in determining the forensic age of an individual. Many authors and researchers have proposed different ways to perform this estimate. The phenomenon of root dentine translucency (RDT) is associated with the increase of age of the individual and has proven to be useful in the estimation of this unknown. The objective of this study was to compare the accuracy in estimating forensic age in three age ranges, by measurement of RDT, using two different methodologies. The protocol consists of taking measurements of the length translucency of dental pieces in a Chilean population (n=94), by direct visual method conventionally used and an indirect digital method based on the capture of a computerized image. The age was estimated applying a table: Three age ranges are preset: 1st range (20-39 ages), 2nd range (40-59 ages) and 3rd range (60-79 ages). The digital method gave better results in the estimation of age in the 1st age range (93%), compared to the visual method that was more assertive in the 2nd and 3rd range (86% and 65%). There are significant differences p=0.001 between the methods for each age range. The difference between the total percentages of successes in the age estimate obtained with both methods are not statistically significant. In conclusion, for age determination in teeth with very little translucency the digital method is recommended, although we must consider that this method tends to underestimate age. In cases of higher RDT we recommend using the visual method; however this method tends to overestimate age. Regardless of the above conclusion, this work shows that both methods are equally effective.


Subject(s)
Humans , Male , Female , Adult , Middle Aged , Aged , Tooth Root/anatomy & histology , Age Determination by Teeth/methods , Dentin/anatomy & histology , Forensic Dentistry/methods , Image Processing, Computer-Assisted , Light
10.
J. appl. oral sci ; 22(3): 165-173, May-Jun/2014. tab, graf
Article in English | LILACS, BBO | ID: lil-711714

ABSTRACT

Objective: To evaluate the impact of the type of root canal preparation, intraradicular post and mechanical cycling on the fracture strength of roots. Material and Methods: eighty human single rooted teeth were divided into 8 groups according to the instruments used for root canal preparation (manual or rotary instruments), the type of intraradicular post (fiber posts- FRC and cast post and core- CPC) and the use of mechanical cycling (MC) as follows: Manual and FRC; Manual, FRC and MC; Manual and CPC; Manual, CPC and MC; Rotary and FRC; Rotary, FRC and MC; Rotary and CPC; Rotary, CPC and MC. The filling was performed by lateral compactation. All root canals were prepared for a post with a 10 mm length, using the custom #2 bur of the glass fiber post system. For mechanical cycling, the protocol was applied as follows: an angle of incidence of 45°, 37°C, 88 N, 4 Hz, 2 million pulses. All groups were submitted to fracture strength test in a 45° device with 1 mm/ min cross-head speed until failure occurred. Results: The 3-way ANOVA showed that the root canal preparation strategy (p<0.03) and post type (p<0.0001) affected the fracture strength results, while mechanical cycling (p=0.29) did not. Conclusion: The root canal preparation strategy only influenced the root fracture strength when restoring with a fiber post and mechanical cycling, so it does not seem to be an important factor in this scenario. .


Subject(s)
Humans , Post and Core Technique/instrumentation , Root Canal Preparation/instrumentation , Root Canal Preparation/methods , Tooth Fractures/etiology , Tooth Root/injuries , Analysis of Variance , Composite Resins/chemistry , Dental Instruments , Dental Stress Analysis , Dentin/anatomy & histology , Materials Testing , Reproducibility of Results , Stress, Mechanical , Surface Properties , Tooth Fractures/physiopathology , Tooth Root/anatomy & histology
11.
Rev. Asoc. Odontol. Argent ; 102(1): 9-20, mar. 2014. ilus, tab
Article in Spanish | LILACS | ID: lil-719590

ABSTRACT

Objetivo: definir si las piezas dentarias con escaso remanente dentinario cervical, con coronas de disilicato de litio, fijadas sobre raíces con postes de resina reforzadas con diferente contenido de fibra de vidrio o con postes de cerámica (circonio), presentan diferente resistencia frente a la acción de fuerzas funcionales paraaxiales simuladas. Como objetivo secundario, analizar el modo de falla del complejo restaurado, a nivel de la corona, el poste o la raíz. Materiales y métodos: se seleccionaron 45 premolares inferiores, recientemente extraidos con un perímetro cervical similar, que fueron divididos al azar en tres grupos de 15 piezas cada uno. Los del grupo 1 recibieron postes de resina reforzada con bajo contenido de refuerzo (ParaPost Fiber White; Coltene-Whaledent, Altstatten, Suiza); los del grupo 2, postes de resina reforzados con alto contenido de fibra de vidrio (FRC postec Plus; Ivoclar-Vivadent, Schaan, Liechtenstein); y los del grupo 3, poste de cristales de zirconia (óxido de zirconio) (CosmoPost, Ivoclar-Vivadent, Schaan, Liechtenstein). Todas las piezas fueron instrumentadas con el sistema mecanizado ProFile (Dentsply-Maillefer, Ballaigues, Suiza): Las áreas cervicales de las piezas fueron cercenadas 1 mm por encima del límite amelo-cementario a fin de evitar la interacción del remanente dentinario con la resistencia que ofrece cada tipo de poste. Los postes fueron cementados siguiendo el mismo protocolo adhesivo. Los grupos 1 y 2 recibieron muñones de resina compuesta. A los del grupo 3 se les inyectó un muñón de cerámica. El tamaño de los muñones de resina compuesta y el de los de cerámica fueron homologados y sobre ellos, tras haberlos impresionado, se construyeron coronas de cerámica libre de metal, inyectadas con disilicato de litio, a las que se les dio forma de caninos superiores. Obtenidas las coronas, se fijaron adhesivamente a las piezas y se incluyeron hasta el límite amelo-cementario en resina epoxi, empleando tubos Bencor


Subject(s)
Ceramics , Crowns , Zirconium/chemistry , Post and Core Technique , Tensile Strength , Dentin/anatomy & histology , Esthetics, Dental , Data Interpretation, Statistical , Tooth Root
12.
Rev. Soc. Odontol. La Plata ; 23(47): 23-28, dic. 2013. ilus, tab
Article in Spanish | LILACS | ID: lil-722390

ABSTRACT

Si bien la dentina y la pulpa tienen marcadas diferencias en su composición y estructura, ambas están tan íntimamente ligadas por su origen embriológico, que cualquier cosa que afecte a la dentina lo hará sobre la pulpa y viceversa. El ejemplo más claro en ese sentido está dado por el líquido intersticial. Este líquido, semejante al plasma pero con menos proteínas, constituye una continuidad entre ambos tejidos y sus efectos hidrodinámicos son muy importantes, tanto en los estados fisiológicos como en los patológicos


Subject(s)
Humans , Dentin/anatomy & histology , Dentin/physiopathology , Dentin/chemistry , Dentin Permeability , Dentin, Secondary/physiology , Dentin/ultrastructure , Dentinal Fluid/physiology , Composite Resins/chemistry , Smear Layer
13.
Braz. dent. j ; 24(3): 230-234, May-Jun/2013. tab, graf
Article in English | LILACS | ID: lil-681862

ABSTRACT

In this study, curved maxillary molar root canals were instrumented with RaCe rotary system to evaluate: 1. the occurrence of canal transportation using a radiographic platform; 2. the action of the instruments on the dentin walls, centering ability and canal enlargement by analysis of digital images; and the percentage of regular dentin surfaces and debris within the canal by histological analysis. Ten mesiobuccal roots of extracted human maxillary molars were embedded in acrylic resin and sectioned at the middle and apical thirds. Root canal shaping was performed using the RaCe rotary system at 250 rpm and 1 Ncm torque. Each instrument set was used five times according to a crown-down technique in the following sequence: 40/0.10, 35/0.08, 25/0.06, 25/0.04, 25/0.02 (working length - WL), 30/0.02 (WL) and 35/0.02 (WL). Each instrument was inserted until resistance was felt and then pulled back, followed by brushing movements towards all canal walls. Each specimen was assessed by three study methods: radiographic platform, digitized image assessment and histological analysis. The radiographic platform showed lack of apical transportation. No statistically significant difference (Wilcoxon test, p>0.05) was found between the middle and apical thirds regarding instrument action on dentin walls, centering ability, area of root canal enlargement, percentage of regular dentin surfaces and debris within the root canal. It may be concluded that RaCe system is a suitable method for the preparation of curved root canals, regarding the maintenance of root canal original path, action on dentin walls, canal enlargement and removal of debris from the root canal lumen.


O objetivo do presente estudo foi verificar a presença de desvio apical por meio da plataforma radiográfica, avaliar a ação dos instrumentos, centralização do preparo e ampliação por meio de imagens digitais do canal e calcular a porcentagem de superfícies regulares e presença de debris por meio da análise histológica. Dez raízes mésiovestibulares de molares superiores extraídos foram incluídos em blocos de resina e seccionados no terço médio e apical. O preparo do canal foi feito empregando o sistema RaCe, a 250 rpm e torque de 1 Ncm. Cada sistema rotatório foi usado 5 vezes no sentido coroa-ápice na seguinte sequência: 40/0.10, 35/0.08, 25/0.06, 25/0.04, 25/0.02 (comprimento de trabalho - CT), 30/0/02 (CT) e 35/0/02 (CT). Cada amostra foi avaliada por 3 métodos de estudo: plataforma radiográfica, imagem digital e análise histológica. A plataforma radiográfica mostrou ausência de transporte apical. Em relação à ação dos instrumentos sobre as paredes dentinárias, centralização do preparo, ampliação, porcentagem de superfícies regulares e presença de debris, o teste estatístico de Wilcoxon não mostrou diferença significativa entre os terços apical e médio. O sistema RaCe se mostrou adequado para o preparo de canais radiculares curvos em relação à manutenção do trajeto original do canal, ação sobre as paredes dentinárias, ampliação e remoção de debris da luz do canal radicular.


Subject(s)
Humans , Dental Pulp Cavity/anatomy & histology , Dentin/anatomy & histology , Root Canal Preparation/instrumentation , Dental Pulp Cavity , Dental Pulp Cavity/surgery , Dentin , Dentin/surgery , Equipment Design , Image Processing, Computer-Assisted/methods , Materials Testing , Molar/anatomy & histology , Rotation , Smear Layer/pathology , Smear Layer , Torque , Tooth Apex/anatomy & histology , Tooth Apex
14.
Rev. Soc. Odontol. La Plata ; 25(46): 21-27, mayo 2013. ilus
Article in Spanish | LILACS | ID: lil-699387

ABSTRACT

Si bien la dentina y la pulpa tienen marcadas diferencias en su composición y estructura, ambas están tan íntimamente ligadas por su origen embriológico, que cualquier cosa que afecte a la dentina lo hará sobre la pulpa y viceversa. El ejemplo más claro en ese sentido está dado por el líquido intersticial. Este líquido, semejante al plasma, pero con menos proteínas, constituye una continuidad entre ambos tejidos y sus efectos hidrodinámicos son muy importantes tanto en los estados fisiológicos como en los patológicos.


Subject(s)
Humans , Dentistry, Operative , Dentin/anatomy & histology , Dentin/physiology , Dentin/ultrastructure , Collagen , Dentin, Secondary , Dentinal Fluid , Dental Cavity Preparation/methods , Dental Pulp/anatomy & histology , Dental Pulp/physiology , Smear Layer
15.
Rev. Soc. Odontol. La Plata ; 25(46): 21-27, mayo 2013. ilus
Article in Spanish | LILACS, BINACIS | ID: biblio-1171305

ABSTRACT

Si bien la dentina y la pulpa tienen marcadas diferencias en su composición y estructura, ambas están tan íntimamente ligadas por su origen embriológico, que cualquier cosa que afecte a la dentina lo hará sobre la pulpa y viceversa. El ejemplo más claro en ese sentido está dado por el líquido intersticial. Este líquido, semejante al plasma, pero con menos proteínas, constituye una continuidad entre ambos tejidos y sus efectos hidrodinámicos son muy importantes tanto en los estados fisiológicos como en los patológicos.


Subject(s)
Humans , Dentin/anatomy & histology , Dentin/physiology , Dentin/ultrastructure , Dentistry, Operative , Smear Layer , Collagen , Dentin, Secondary , Dentinal Fluid , Dental Pulp/anatomy & histology , Dental Pulp/physiology , Dental Cavity Preparation/methods
16.
Arq. odontol ; 49(1): 12-18, 2013. ilus
Article in Portuguese | LILACS, BBO | ID: lil-698339

ABSTRACT

Objetivo: Avaliar a atenuação da luz através de diferentes espessuras de resina composta e a profundidade de polimerização. Materiais e Métodos:Foram utilizadas as resinas Opallis e Llis de esmalte (E) e de dentina (D), na cor A3. Para atenuação da luz foram empregadas matrizes metálicas (6,0mm de diâmetro e espessuras de 0,5, 1,0, 1,5 e 2,0mm), as quais foram posicionadas na janela de leitura do radiômetro e a intensidade de luz medida antes e após a interposição do material. A profundidade de polimerização foi realizada seguindo os procedimentos recomendados na ISO 4049 (2000). Os dados foram submetidos à ANOVA e teste de Tukey com α=5%. Resultados: Os resultados de atenuação mostraram que as resinas Opallis e Llis diferiram estatisticamente em todas as espessuras. Na comparação entre materiais, em 0,5mm houve diferença estatística significante apenas entre Opallis E e Llis D e entre Llis E e Llis D. Em 1,0mm não houve diferençaestatística significante entre Opallis E e Llis E e entre Opallis D e Llis D; em 1,5mm somente Opalllis D e LlisD não diferiram estatisticamente. Para 2,0mm ocorreram diferenças estatística significantes entre Opallis E eLlis E. Para profundidade de polimerização não houve diferença estatística significante entre Opallis E e Llis E e entre Opallis D e Llis D.Conclusão: Maiores valores de atenuação da intensidade de luz são observadosem resinas de dentina em ralação as de esmalte, assim como em maiores espessuras de material. Resinascompostas de dentina apresentaram menor profundidade de polimerização que as de esmalte.


Subject(s)
Dentin/anatomy & histology , Composite Resins/analysis , Light/adverse effects , Microscopy, Polarization , Polymerization
17.
J. appl. oral sci ; 20(2): 151-156, Mar.-Apr. 2012. ilus
Article in English | LILACS | ID: lil-626413

ABSTRACT

Direct composite resin restorations have become a viable alternative for patients that require anterior restorative procedures to be integrated to the other teeth that compose the smile, especially for presenting satisfactory esthetic results and minimum wear of the dental structure. Technological evolution along with a better understanding of the behavior of dental tissues to light incidence has allowed the development of new composite resins with better mechanical and optical properties, making possible a more artistic approach for anterior restorations. The combination of the increasing demand of patients for esthetics and the capacity to preserve the dental structure resulted in the development of different incremental techniques for restoring fractured anterior teeth in a natural way. In order to achieve esthetic excellence, dentists should understand and apply artistic and scientific principles when choosing color of restorative materials, as well as during the insertion of the composite resin. The discussion of these strategies will be divided into two papers. In this paper, the criteria for color and material selection to obtain a natural reproduction of the lost dental structures and an imperceptible restoration will be addressed.


Subject(s)
Humans , Composite Resins/chemistry , Dental Restoration, Permanent/methods , Esthetics, Dental , Prosthesis Coloring/methods , Color , Dental Enamel/anatomy & histology , Dentin/anatomy & histology , Reproducibility of Results , Treatment Outcome
18.
Article in English | IMSEAR | ID: sea-141221

ABSTRACT

Context: During fixed orthodontic therapy, when the stress levels in the periodontal ligament (PDL) exceedsan optimum level, it could lead to root resorption. Aims: To determine an apical stress incident on the maxillary central incisor during tooth movement with varying cemental and periodontal ligament thickness by Finite Element Method (FEM) modeling. Settings and Design: A three dimensional finite element model of a maxillary central incisor along with enamel, dentin, cementum, PDL and alveolar bone was recreated using EZIDCOM and AUTOCAD software. ALTAIR Hyper mesh 7.0 version was used to create the Finite Element meshwork of the tooth. This virtual model was transferred to Finite Element Analysis software, ANSYS where different tooth movements were performed. Materials and Methods: Cemental thickness at the root apex was varied from 200 μm to 1000 μm in increments of 200 μm. PDL thickness was varied as 0.24 mm and 0.15 mm. Intrusive, Extrusive, Rotation and Tipping forces were delivered to determine an apical stress for each set of parameters. Results: Results indicated that an apical stress induced in the cementum and PDL, increased with an increase in cementum and PDL thickness respectively. Apical stress induced in the cementum remained the same or decreased with an increase in the PDL thickness. Apical stress induced in the PDL decreased with an increase in the cementum thickness. Conclusion: The study concluded that the clinical delivery of an orthodontic forces will cause stress in the cementum and PDL. Hence, it is necessary to limit the orthodontic force to prevent root resorption.


Subject(s)
Adolescent , Adult , Alveolar Process/anatomy & histology , Biomechanical Phenomena , Computer Simulation , Dental Cementum/anatomy & histology , Dental Enamel/anatomy & histology , Dental Pulp/anatomy & histology , Dentin/anatomy & histology , Finite Element Analysis , Humans , Imaging, Three-Dimensional/methods , Incisor/physiology , Maxilla/physiology , Models, Biological , Orthodontic Extrusion/methods , Periodontal Ligament/anatomy & histology , Rotation , Stress, Mechanical , Tooth Apex/physiology , Tooth Movement Techniques/methods
19.
Pesqui. vet. bras ; 32(1): 93-95, Jan. 2012. ilus
Article in English | LILACS | ID: lil-614736

ABSTRACT

The teeth of Azara's agouti (Dasyprocta azarae) were described macroscopically in order to provide biological data on one of the largest wild rodents of the Americas. Radiography was taken on six heads and the teeth were described. Enamel surrounds the coronal dentin, projects to the roots and is present as parallel inner laminae in buccolingual direction. The dentin is located among the enamel laminae and surrounds the pulp horns. The cementum is located internally to the enamel laminae. On the lingual surface, the cementum and dentin are the outer elements.


Os dentes de cutias (Dasyprocta azarae) foram descritos macroscopicamente para fornecer informações sobre um dos maiores roedores das Américas. Radiografias foram realizadas em seis cabeças, e os dentes foram descritos. O esmalte envolve a dentina coronal e se projeta até a raiz e é presente como lâminas dispostas paralelamente em direção vestíbulolingual. A dentina é localizada entre as lâminas do esmalte e envolve os cornos pulpares. O cemento é localizado internamente às lâminas de esmalte. Na superfície lingual, o cemento e a dentina são os elementos mais externos.


Subject(s)
Animals , Tooth/anatomy & histology , Tooth , Dentin/anatomy & histology , Molar/anatomy & histology , Bicuspid/anatomy & histology
20.
Rev. Assoc. Paul. Cir. Dent ; 66(1): 24-29, jan.-mar. 2012.
Article in Portuguese | LILACS, BBO | ID: lil-654817

ABSTRACT

A radiopacidade é um pré-requisito imprescindível para materiais utilizados como base eforramento de restaurações. Possibilita que o profissional identifique a presença do material,permitindo a diferenciação com a estrutura dental adjacente. Objetivo: O objetivo desse estudofoi avaliar a radiopacidade de materiais de base e forramento disponíveis para uso clínico ecomparar com a radiopacidade do esmalte e dentina. Materiais e Métodos: Foram analisados:13 marcas comerciais de cimento de ionômero de vidro, 1 amálgama e 1 resina composta. Foramconfeccionados três corpos de prova com espessura de 1 mm para cada cimento de ionômerode vidro e para cada material restaurador. Obteve-se 1 espécime, com 1 mm de espessura, deesmalte/dentina através de um corte transversal da coroa dental. As tomadas radiográficas foramfeitas com o sistema digital ~VG 5000 Kodak e tempo de exposição de 0,32 segundos. Asimagens obtidas foram trabalhadas no programa Image Tool® obtendo-se os valores médiosde cinza. A análise estatística ANOVA, seguida pelo teste de Tukey (p 0,05) detectou diferençasconsideráveis. Resultados: Alguns cimentos de ionômero de vidro apresentaram radiopacidadeinferior à da dentina e inadequada segundo as normas ISO9917, sendo considerada insuficientepara um correto diagnóstico radiográfico. Conclusão: Conclui-se que existem cimentos de ionômerode vidro indicados como base e forramento de restaurações disponíveis para aquisiçãocom radiopacidade imprópria para serem detectados radiograficamente.


The radiopacity is an essential prerequisite for materiais used as a base liner and restorationsand allows the professional to identify the presence of the material allowing the differentiationfrom the adjacent tooth structure. Objectives: The aim of this study was to evaluate the radiopacityof base and liner materiais available for clinical use and compare to the radiopacity ofenamel and dentin. Materiais and methods: 13 glass ionomer cements, one resin composite andone amalgam were evaluated. Three specimens with 1 mm thickeness for each glass ionomercement and restorative material were prepared. Cross section was made from a third molardental crown to obtain enamel and dentin specimen with thickness of 1 mm. Radiographswere taken with digital system Kodak RVG5000 and exposure time of 0.32 seconds. The imageswere analysed through the programe Image Tool" to obtain the mean grey values. Statisticaltest ANOVA followed by Tukey test (p<0.05) detected considerable differences. Results: Someglass ionomer cements presented radiopacity lower than dentin, considered insufficient for acorrect radiographic diagnosis, according to ISO 9917. Conclusion: It was concluded that somebase and liner materiais available for purchase have an inadequaderadiopacity to be detectedradiographically.


Subject(s)
Glass Ionomer Cements/therapeutic use , Dentin/anatomy & histology , Radiography, Dental, Digital/methods
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